Coding

Part:BBa_K2637018:Design

Designed by: Jiangnan Xie   Group: iGEM18_Tianjin   (2018-10-05)


Fluc KaiA is one of the essential proteins in the circadian rhythm of cyanobacteria. In subjective dawn, KaiA combines with KaiC and activates its kinase activity, resulting in phosphorylation of two sites on KaiC, which subsequently serves as the signal of KaiABC oscillator to regulate physiological activities in cyanobacteria. We have optimized it in yeast.

Our basic parts range from BBa_K2637001 to BBa_K2637018 and BBa_K2637053 to BBa_K2637059. Our composite parts range from BBa_K2637021 to BBa_K2637044 and BBa_K2637047 to BBa_K2637052. Our part collection include all our basic parts except for BBa_K2637018 and all composite parts.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1314


Design Notes

We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.



Source

123

References